The hESC Core Facility
The hESC Core Facility at Children’s Hospital Boston provides training in stem cell culture, regulations, and separation of funding requirements to the Hospital's stem cell community. We also maintain the internal Children's Hospital Boston stem cell registry and bank, expand, perform quality control for, and ship Children's Hospital Boston hESC lines to laboratories interested in these lines.
Our research efforts focus on directed differentiation of pluripotent stem cells. We combine fluorescent imaging and other stem cell based assays with high-throughput chemical screening approaches in order to identify novel agents and pathways that affect cell fate. Our goal is to derive blood stem cells from pluripotent stem cells.
hESC CORE FACILITY STAFF
Thorsten Schlaeger, PI
Xiao Guan, Postdoc.
Odelya Hartung, Research Assistant
Phil Manos, Research Assistant
Iris Huo, Research Assistant
Mam Ratanasirintrawoot, PhD Student
Sumon Datta, former Research Assistant
Justine Miller, former Research Assistant
Leah Boyer, former Research Assistant
Holly Young, former Research Assistant
Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells. Chan EM, Ratanasirintrawoot S, Park IH, Manos PD, Loh YH, Huo H, Miller JD, Hartung O, Rho J, Ince TA, Daley GQ, Schlaeger TM. Nat Biotechnol. 2009 Oct 11.
Improved electrospray ionization efficiency compensates for diminished chromatographic resolution and enables proteomics analysis of tyrosine signaling in embryonic stem cells. Ficarro SB, Zhang Y, Lu Y, Moghimi AR, Askenazi M, Hyatt E, Smith ED, Boyer L, Schlaeger TM, Luckey CJ, Marto JA. Anal Chem. 2009 May 1;81(9):3440-7.
Derivation and maintenance of human embryonic stem cells from poor-quality in vitro fertilization embryos. Lerou PH, Yabuuchi A, Huo H, Miller JD, Boyer LF, Schlaeger TM, Daley GQ. Nat Protoc. 2008;3(5):923-33.
Inducible endothelial cell-specific gene expression in transgenic mouse embryos and adult mice. Deutsch U, Schlaeger TM, Dehouck B, Döring A, Tauber S, Risau W, Engelhardt B. Exp Cell Res. 2008 Apr 1;314(6):1202-16.
Enhanced plating efficiency of trypsin-adapted human embryonic stem cells is reversible and independent of trisomy 12/17. Chan EM, Yates F, Boyer LF, Schlaeger TM, Daley GQ. Cloning Stem Cells. 2008 Mar;10(1):107-18.
Science aside: the trajectory of embryonic stem cell research in the USA. Schlaeger TM, Lensch MW, Taylor PL. Drug Discov Today. 2007 Apr;12(7-8):269-71.
Pluripotent stem cells and their niches. Lensch MW, Daheron L, Schlaeger TM. Stem Cell Rev. 2006;2(3):185-201. Review.
- ISSCR hESC Guidelines
- NAS hESC Guidelines
- NIH Stem Cell Guidelines
- NIH Stem Cell FAQs
- Obama 2009 Policy (PDF)
- OMB Circular A-21
- OMB Circular A-122
- Human Subject Protection
- NIH Stem Cell Registry
- NIH Cell Line Submission
- MA Stem Cell Act