The hESC Core Facility

hESC Training Opportunities

NEW! APPLY ON-LINE FOR THE HSCI hESC TRAINING COURSE
For direct access to the on-line registration page, go to www.macusa.net/CHB. Harvard and Harvard affiliated researchers who are interested in taking the HSCI funded hESC cell culture course may now apply on line.

Please read the FAQ’s below before submitting your on-line application. This will help you determine your eligibility and insure that the course is appropriate for your needs.

Once you have verified that you meet the eligibility guidelines, follow the Course Signup Instructions below.

Note: if you have well-established hESC protocols that you want to share please send them (text file plus images) to Dr. Thorsten Schlaeger, Head of the hESC Core. Dr. Schlaeger will review the material and if appropriate enter it into the hESC protocol database (see FAQs for details on how to access the database).

COURSE SIGN-UP INSTRUCTIONS

(1) Go to http://www.macusa.net/CHB
(2) Log in (username= “new user” password= “hesccore”)
(3) Complete the form (all fields must be completed; be sure to enter your e-mail correctly – this will be your user name for future log-ins and the hESC Core’s only line of contact to you.)
(4) IMPORTANT: Click “create account” button (failing to do so means the information you just entered will not be stored; be sure to check if it worked: the text prompting you to do so will disappear and the button label should switch to “update password”)
(5) Select a course (or several courses – there is a limit of 2 trainees per course; you can see the number of applicants per course)
(6) Log-out (You may log in again at any time, e.g. to change your selections, to inactivate your account, or to confirm your participation in a particular course if prompted to do so in the course invitation e-mail)

FAQs

Who is eligible to participate?

Any member of a laboratory affiliated with Harvard University / the Harvard Stem Cell Institute / Harvard Medical School who has at least basic knowledge of sterile technique and mammalian cell culture. NOTE: as a general rule, ONLY ONE APPLICANT IS ACCEPTED PER LAB. Trainees are expected to teach their lab mates the hESC culture techniques they learned in the course.

How do I create an account?

Follow the Signup Instructions below, complete the top part of the form, and click the “Create Account” button. Your e-mail will be your log-in user name.

How much does the course cost?

There is no fee: the Harvard Stem Cell Institute has funded the training through a grant to the CHB hESC core. As noted above, we do expect that applicants trained from a given lab will arrange to train their lab mates in the procedures they have learned.

How long is the course?

One week (4 half-days Mo, Tu, We, Fr).

What topics are covered?

Culture on feeders and feeder-free culture in chemically defined media; mechanical and enzymatic clump passaging; single cell passaging and lentiviral gene transduction; alkaline phosphatase staining and pluripotency marker immunofluorescence; embryoid body formation; feeder cells (MEFs).

I plan to work with IPS cells – should I take the hESC training course?

hIPS cells and hES cells are essentially cultured the same way. This course will teach you how to grow and manipulate these cells.

What happens if a course is oversubscribed?

Multiple factors are taken into consideration, including the timing of your application, PI’s HSCI affiliation, and the number of past trainees from the same laboratory. If you are eligible to participate (see above) but did not get into any of the courses you selected you should log in again and select additional course dates.

Why should I choose more than one course?

These courses are in high demand and tend to fill up quickly. Also, in rare occasions, a course may have to be canceled. You should always choose multiple dates (as long as you know they fit your schedule) – you may choose up to 10 dates (you can always have up to 10 active selections). Choose the dates carefully – if you repeatedly decline offers, fail to respond to invitations, or do not show up to a course, you and your lab may be restricted from further enrollment. To avoid that possibility, as soon as you know that you will be unable to participate in a course for which you have applied, please un-select courses or mark your account as “no longer interested”, as appropriate.

Should I choose a course that already has several applicants?

While your chances are best if you select courses when they have less than two applicants, this does not mean you should never choose such a course, for example as a backup. Space may become available if applicants that are ahead of you later choose to un-select the course, decline an invitation, or be invited to and complete an earlier course.

All available courses are full – what should I do?Choose up to 10 of the least subscribed-to courses that fit your schedule, and keep checking back. We are adding course dates on a regular basis. You may also e-mail Dr. Schlaeger at schlaeger@enders.tch.harvard.edu.
When and how will I know if I will be admitted to a course?

You will receive an e-mail notification at least one week ahead of the course. You must confirm your participation by logging into your account and confirming/declining by using the buttons at the bottom of the form.

What if something comes up that prevents me from participating?

Please choose your dates carefully – if you repeatedly decline offers, fail to respond to invitations, or do not show up despite confirmation you and your lab could be restricted from further enrollment. To avoid that possibility, as soon as you know that you will be unable to participate in a course for which you have applied, please un-select courses or mark your account as “no longer interested”, as appropriate.

How do I change my password or e-mail?

Once you have created an account you can change the password by entering a new password, then clicking “Update Password”. You cannot change your username (you won’t be able to log in again if you alter the e-mail address you originally entered.) If your old e-mail is no longer working please send your new e-mail to schlaeger@enders.tch.harvard.edu.

How do I access the hESC protocol database?

You either set up an account and log in as described above or you log in using the following username/password: protocols / hesccore.

Can I submit hESC protocols?

Yes, you are encouraged to submit well established protocols – please send them to schlaeger@enders.tch.harvard.edu (text+images OK). Protocols should be as detailed as possible.

How do I contact the HSCI hESC Core facility directly?

By e-mail: schlaeger@enders.tch.harvard.edu.

Giving